Scientific Volume Imaging B.V.

It is our pleasure to mention Scientific Volume Imaging as a user example for our GATTA-Beads (soon available). SVI used ATTO 647N labeled nanobeads from GATTAquant for high resolution STED imaging. Images were acquired on a Leica TCS SP8 STED 3X system. By acquiring optimally sampled z-stacks, SVI was able to reconstruct 3D PSFs by averaging over multiple GATTA-Beads, followed by a PSF distilling process in the SVI Huygens Software. The distilled PSF was then used for deconvolution in the Huygens Professional Software. This is one of the first examples where a 3D STED PSF was accurately distilled from bead data and demonstrated to be beneficial to the STED deconvolution result.

GATTAquant_647N_deconv_MIP_STED_640x480-small.gif

Images were acquired with the Leica TCS SP8 STED 3X system at VUmc, Amsterdam, The Netherlands, and were processed with the Huygens Professional software.
Acquired by: Lino Miltenburg (SVI) and Jeroen Kole (VUmc)
Processed by: Remko Dijkstra (SVI)

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