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GATTA-Beads

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Small, bright and cutting-edge. Experience the highest brightness density in the world.

Fluorescent beads are important microscopy tools which can be used for calibration of microscopes, particle tracking or quantitative determination of point spread functions especially in STED microscopy. Particularly for the last point a small structure size keeping a high brightness is an immense advantage. Products from the GATTA-Bead series fulfill this requirement perfectly with a diameter of only 23 nm. Additionally, they show a clearly improved homogeneity and flexibility compared to other beads. We offer GATTA-Beads in the colors red (ATTO 647N), orange (ATTO 594), green (ATTO 542) and blue (Oregon Green 488). Moreover, we offer alternative dyes which are indicated on the table below.

On request we can also design special solutions for your specific requirements. All samples will be delivered on a sealed glass slide which you can comfortably put directly on your microscope.

Fields of Application:

  • Setup calibration
  • 2D STED measurements
  • 3D STED PSF distillation and deconvolution
  • Tracking and drift-correction


Advantages:

  • Size: d = 23 nm
  • Brightness: the highest brightness density
  • Homogeneity: super small size and intensity distribution
  • Flexibility: change color or add chemical groups

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3D stack of GATTA-Beads R measured at VUmc, Amsterdam, on a Leica TCS SP8 STED 3X. 3D PSF distillation and rendering by Scientific Volume Imaging using the MIP renderer in Huygens Professional

New super-resolution Buttonproduct properties of GATTA-Beads

Bead R Bead O Bead G Bead B
Color red orange green blue
Available fluorophores ATTO647N ATTO 594 ATTO 542 Oregon Green 488
Alternative fluorophores Abberior STAR 635P - Cy3 or Alexa Fluor 555 Alexa Fluor 488 or ATTO 490LS
Recommended laser source 630-650 nm 565-610 nm 515-540 nm 480-505 nm
Diameter 23 nm 23 nm 23 nm 23 nm
Surface density ≈ 1/µm² ≈ 1/µm² ≈ 1/µm² ≈ 1/µm²
Price (sample on glass slide) 300,-€ 300,-€ 300,-€ 300,-€
Price (sample in solution) 500,-€ 500,-€ 500,-€ 500,-€

fluorophore properties

 Fluorophore Our notation Excitation / nm
Emission / nm
ATTO 647N R  647
664
Abberior Star 635P R  635
651
ATTO 594 O 594 626
ATTO 542 G 542 562
Cy3 G 554 568
Alexa Fluor 555 G 555 580
Oregon Green 488 B 488 526
Alexa Fluor 488 B 488 525
ATTO 490LS B 490 658

 

 

product sheet
Full size super-resolution Images
Publications

J.J. Schmied, R. Dijkstra, M. Scheible, G. M. R. De Luca, J. J. Sieber, GATTAquant GmbH, Scientific Volume Imaging (SVI), Leica Microsystems (2016): Measuring the 3D STED PSF with a new Type of Fluorescent Beads Science Lab

References / Experience Reports

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contact

GATTAquant GmbH
Karlstr. 82
DE-38106 Braunschweig

Phone: +49 (531) 391 552 55

Fax: +49 (531) 388 249 16

INFO@GATTAQUANT.COM

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